A little bit of inorganic phosphate can go a LONG way! especially if you add pure carbon and nitrogen sources in excess like I am doing.What I am getting at is why the need for more GFO then he was using before, it the amount of phosphates if teh L-aspartic acid didn't add any?
Before adding these C and N sources, they were probably limited and it didn't matter how much phosphate was present (in terms of bacterial growth). Phosphate alone will do nothing if no other important metabolic compounds are present. But once I added them then the phosphate mattered ALOT!